Eficacia in-vitro de un colutorio elaborado con aceite esencial de la hoja de ishpingo Ocotea quixos (Lam.) Kostern. ex O.C.Schmidt y clavo de olor Syzygium aromaticum (L.) Merr. & L.M. Perry

Abstract

Considering the CMB of the ishpingo essential oil (0.26%) and the essential oil of clove (1.56%) against the Streptococcus mutans and the Streptococcus pyogenes, applying the dilution method in liquid medium we developed a mouthwash. In order to determine the effectiveness of our mouthwash, prepared in vitro, we introduce in the brain and heart infusion agar strains of Streptococcus mutans ATCC 25175 and ATCC 19615 Streptococcus pyogenes. Using the diffusion method on solid medium, we placed 25 μL of our mouthwash and commercial mouthwashes on discs of filter paper and we incubated at 37 °C for 24 hours S. pyogenes strains and 48 hours for S. mutans strains. According to the results, the mouthwash we produced growth inhibition zones of bacterial colonies. For the statistical analysis of the samples we used the nonparametric Kruskal Wallis test on Statistix 8.0 software and determined that there are differences between our mouthwash and the commercial products