Evaluación de la actividad antimicrobiana de extractos proteicos inhibidores de tripsina provenientes de semillas de sangorache (Amaranthus hybridus L.)

Abstract

This study evaluated the antimicrobial activity of protein extracts from seeds of sangorache (Amaranthus hybridus L.), against pathogenic bacteria Serratia marcescens, Pseudomonas aeruginosa, Streptococcus group G and C, Micrococcus luteus and Escherichia coli. The obtain the protein extracts, previously degreased crushed seeds were used, which were subjected to purification techniques such as ultrafiltration with centrifugation and enzymatic immobilization to obtain the extracts with three levels of purity. After obtaining the extracts, the antimicrobial activity was evaluated using the disk diffusion technique in agar, a test that allowed to measure the susceptibility in vitro. The results were reported based on the size of the growth inhibition halo formed by the bacteria versus the extracts and was interpreted quantitatively through a completely randomized and qualitative design by comparing the results with tables from the CLSI manual. The best results were obtained with the semi-purified and purified extracts, since they inhibited the growth of E. coli, S. marcescens and Streptococcus group C and G, but treatment 7 that showed an inhibition halo of 14.57 mm, much greater than the rest of treatments. Based on the CLSI manuals, Escherichia coli was in the intermediate category compared to the semi-purified and purified extracts, and the rest of the strains were cataloged as resistant to the three types of protein extracts.