EVALUACIÓN DE TRES NIVELES DE NITRÓGENO EN MEDIO DE CULTIVO, EN LAS FASES DE ENRAIZAMIENTO in vitro Y ADAPTACIÓN A SUSTRATO DE Rubus glaucus (BENTH)

Abstract

This work was developed in canton Rumiñahui, Pichincha province, Ecuador. The objective of this work was to evaluate three nitrogen concentrations of Murashige and Skooog salts (MS) in the culture medium, during the stages rooting in vitro and substrate adaptation of Rubus glaucus. The 4.88mM dose of nitrogen in the medium had the highest number of roots with an average of 3.72 roots per plant, increased root length of 1.8cm long, percentage of rooting in vitro and in substrate, with an average 78% and 100% respectively. Also resulted in lower levels of plant stress, measured in the index of chlorophyll content and photosystem II activity or variable fluorescence. The results show that the half of nitrates was a major factor in increasing rooted plants.