Identificación de metabolitos producidos en la biodegradación de carbamazepina por hongos

Abstract

Carbamazepine (CBZ) is one of the drugs that is found contaminating the water sources of Ecuador, affecting the entire biotic community that surrounds it. Currently, as a measure for bioremediation, the use of fungi arises, which have enzymes such as peroxidases and laccases, which allows them to have a high removal capacity compared to bacteria. Within the degradation process, the formation of intermediate metabolites must be taken into account since it would determine the efficiency of the degradation. So, a bibliographic compilation was carried out in data sources such as Google schoolar, Scielo, ScienceDirect and Scopus from the years 2019 to 2024 about methodologies that are used for the identification of secondary metabolites that are generated after the degradation of (CBZ) for which initially, the fungus Pleurotus ostreatus was identified bibliographically, which has a degradative capacity of 99% and with this an experimental design related to a degradation test was proposed where factors such as: pH from 5 to 6 and temperature of 20°C. Through the bibliographic review, the Thin Layer Chromatography (TLC) method was chosen, with which the methodology for carrying out the test was proposed that will allow the identification of intermediate metabolites obtained during the biodegradation of CBZ considering that the tests will be carried out in the facilities from the Salesian Polytechnic University.