Determinación por taxonomía molecular de hongos contaminantes persistentes en el laboratorio de Biotecnología Vegetal

Abstract

Contamination of in vitro explants is a difficult problem to deal with, since disinfection protocols address general qualities of microorganisms without determining the specific characteristics that can make them resistant to traditional methods. For this reason, in order to determine their taxonomy, three fungi isolated from in vitro cultures were identified using molecular extraction, amplification and sequencing techniques, obtaining the species: R. stolonifer, E. italicum and T. hirsuta.