Estandarización de un protocolo de qPCR para la identificación de la enfermedad de la mancha blanca en camarones de piscina de las provincias del Guayas y Manabí

Abstract

The present thesis work had the purpose of carrying out the standardization of a qPCR protocol for the identification of white spot disease in shrimp farms in the provinces of Guayas and Manabi. Positive samples of shrimp exhibiting characteristic signs and symptoms of the disease of interest were used, along with samples taken directly from the pools of the mentioned provinces. The procedures that were carried out were the extraction of the DNA of each sample by different methods; DNA quantification by Qubit and NanoDrop; evaluation of DNA integrity by agarose gel electrophoresis; evaluation and selection of primers; qPCR amplification and protocol evaluation. The results obtained indicate that it was possible to adequately standardize the protocol for the identification of the white spot disease, which was evaluated with a dilution test to know the sensitivity of the protocol in the analysis of this disease presented by pool shrimp. Therefore, it is concluded that White spot detection is possible using a qPCR protocol.