Estudio fitoquímico e identificación molecular de las especies del género Bomarea del norte de los Andes del Ecuador, mediante el sistema BARCODE.

Abstract

Alstroemeriaceae is a family of monocotyledonous plants whose most recognized genera are Alstroemeria and Bomarea, the latter being the only one present in Ecuador with colorful bell-shaped flowers that distinguish it. In this research, a molecular analysis of the species located in the north of the Andes of Ecuador was carried out with the collection of 41 specimens from which DNA was extracted from young leaves with the Doyle & Doyle technique, the chloroplastic region rpoC1 was expanded using the conventional PCR technique using the Phire Plant Direct PCR Master Mix kit, the products obtained were sequenced using the Sanger method and a phylogenetic construction analysis was performed with Mega 7.0 software, while for analysis the DIVA GIS program was used; screening assays and spectrophotometric techniques were used in the phytochemical evaluation of secondary metabolites present. Among the results obtained was the formation of a phylogenetic tree with six distinct clades between species, Bomarea multiflora presented a wide range of distribution along the interandean alley and phytochemical analysis determined the presence of phenolic compounds where It was concluded that Bomarea linifolia is the species with the greatest antioxidant capacity in the reduction of DPPH radicals with IC50 of 251,363 g/mL and 3,729 mg GAE/ g of total phenols which places as a species for potential for potential future anti tumor studies.