Evaluación de diferentes concentraciones de ácido indolbutírico (IBA) y 6-bencilaminopurina (BA) en la propagación in vitro de Espeletiopsis muiska

Abstract

This study evaluated the effect of different concentrations of the growth regulators indolebutyric acid (IBA) and benzylaminopurine (BA) on the germination and initial in vitro development of Espeletiopsis muiska, a species endemic to the Ecuadorian paramos. Nine treatments with varying combinations of IBA and BA were applied to achenes, analyzing three main variables: germination percentage, number of shoots, and root length. The data were processed using Kruskall-Wallis nonparametric statistical analysis, Dunn's post hoc test, and chi-square tests, using R. The results reveal that the most effective treatment is the one that uses a balance of IBA and BA, with high germination percentages, high number of shoots, and significantly higher rooting. These findings suggest that a balanced combination optimizes the in vitro development of E. muiska, representing an important advance for its propagation and ex situ conservation.