Validación de un protocolo para la detección molecular de Rickettsia SPP. mediante la técnica de reacción en cadena de la polimerasa

Abstract

Serological diagnosis has been considered the gold standard for the diagnosis of rickettsial diseases. However, these assays present various limitations regarding their specificity and ability to detect infections in early stages. This study aims to validate an endpoint PCR protocol for the molecular detection of Rickettsia spp. To this end, the specificity of the primers was determined using bioinformatic tools. The sensitivity of the protocol was determined through serial dilutions of the positive control. Finally, validation was carried out using blood samples from canines and arthropod vectors, transmitters of the disease. One positive sample was obtained in the validation of arthropod vectors. However, no positive results were obtained from the canine blood samples.