Validación de una especie vegetal como cultivo trampa de la Familia Asteraceae para la multiplicación de micorrizas arbusculares nativas en un sistema in vitro autotrófico

Abstract

The evaluation of the efficacy of a plant species of the Asteraceae family as a trap culture for the multiplication of AMF under autotrophic in vitro conditions was the purpose of this study. To control microbial growth and maximize mycorrhizal development, an in vitro culture system with different concentrations of antibiotics (gentamicin and streptomycin) was used. The results obtained showed that Tagetes patula and Bidens pilosa are more susceptible to colonization by AMF compared to Tridax procumbens. With a high density of mycorrhizal structures. For the multiplication of these mycorrhizas and the growth of the plant in vitro, the concentration of gentamicin at 100% was the most effective. This discovery suggests that the development of symbiosis between the plant and mycorrhizal fungi can be facilitated by effectively controlling the growth of unwanted microorganisms, using gentamicin at the appropriate concentration. Furthermore, the above-mentioned specimens are presented as an excellent option for use as a trap culture in the production of mycorrhizal inocula on a large scale. This method offers a promising solution for the production of AMF inocula under controlled conditions, which could have important applications in sustainable agriculture and ecosystem restoration. However, it was determined that the concentration of gentamicin must be carefully adjusted to avoid phytotoxic effects and ensure the effectiveness of the in vitro system.