Desarrollo de un protocolo de cultivo celular de queratinocitos in vitro para los laboratorios de ciencias de la vida de la Universidad Politécnica Salesiana

Abstract

Internationally, there are standardized guidelines for cell culture, but in Ecuador there is no protocol adapted to the resources available in the country. Recognizing the importance of cell culture in biomedical development for the treatment of various skin pathologies, the life sciences laboratories of the Universidad Politécnica Salesiana, Quito, have taken the initiative to develop a protocol for in vitro keratinocyte cell culture. This protocol uses the immortalized human keratinocyte cell line (HaCaT), thus avoiding infringing bioethical codes, such as the Helsinki Code. HaCaT cell lines have a wide variety of phenotypic characteristics, making them particularly suitable for this type of culture. The implementation of this protocol has resulted in a high proliferation of keratinocytes, demonstrating its efficacy and obtaining a positive resolution.