Protocolo para el desarrollo de una forma farmacéutica compuesta por extracto alcohólicos de cuatro especies vegetales en la prevención y/o tratamiento de mastitis en ganado vacuno

Abstract

There are reports of ancestral knowledge of medicinal plants for veterinary use in the community of Paquiestancia located in the Cayambe Canton. Through the exploratory study, information was obtained on the use of four plants: elderberry, mallow, lechango and nightshade, which are used in the form of plasters for the prevention of mastitis in cattle. There are no reports in the literature regarding the preparation of pharmaceutical products for external use in veterinary medicine based on these plants, so the objective of this work is to design a protocol for the development of a pharmaceutical form based on alcoholic extracts of the species Cynanchum microphyllum Kunth, Cestrum peruvianum Willd. former Roem. & Schult, Solanum nigrum, Malva sylvestris, obtained from leaves and stems for the prevention and/or treatment of bovine mastitis. The protocol proposal contemplated four stages. Stage I: includes the study of the art for the selection of semisolid pharmaceutical forms for external use in the veterinary field. Stage II: formulation and preparation of the bases of the selected pharmaceutical forms. Stage III: quality control analysis of pharmaceutical forms under normal conditions and under conditions of forced stability. Stage IV: selection of the representative GEL pharmaceutical form from among the pharmaceutical forms selected in stage I, based on the results obtained in stage III. Stage IV also includes the application of the Gel base product to cattle, finding optimal results with respect to the absence of adverse effects during the 7 days that the study lasted in the two days of milking. In addition, the easy application and removal of the product with water, a friendly procedure for the community member in the milking activity, were identified. In this work, a preformulating of the gel is left considering 3% of active ingredient.