Revisión del género Bomarea del Ecuador mediante información molecular a través del sistema barcode

Abstract

The Alstroemeriaceae family has several genera, the best known of which are Alstroemeria and Bomarea, the latter being the genus that is mostly distributed in areas of the Andes Mountain range. In the present investigation a molecular study of species located in the inter-Andean region of Ecuador was carried out. Plant samples collected in the provinces of Pichincha and Tungurahua were processed for molecular analysis. DNA was extracted with the Doyle & Doyle technique and commercial kit, where the samples were amplified the chloroplastic regions matK and ycf1 by conventional PCR technique using the Platinum Direct PCR Universal Master Mix kit, the sequences used were obtained from the Tropicos website. To continue with the process, the sequences were cleaned and aligned, the phylogenetic trees of the matK, rbcL and rpoC1 markers were constructed using MEGA 11 software, while for the biogeographic analysis the DIVA GIS program was used to demonstrate the distribution and concentration of the species of the Bomarea genus in the interAndean region of Ecuador. Among the results obtained, the 3 phylogenetic trees with their respective markers were presented, where the phylogenetic tree of matK showed greater discrimination among the other markers.