Cultivo del microcrustáceo Moina sp como alternativa alimentaria para el cultivo en laboratorio de pez cebra (Danio rerio) en Quito-Ecuador

Abstract

The present work seeks to contribute to scientific knowledge through the cultivation of the microcrustacean Moina sp as a food alternative for the laboratory culture of zebrafish (Danio rerio) in Quito-Ecuador, to fulfill this purpose, a culture protocol will be established, using 9 plastic containers of 2L capacity, each bottle supplied with 1.5L of conditioned water at a temperature of (20±2) and pH of 7, each treatment was fed with different diets: Spirulina powder at a concentration of 0.001 g/ mL, Saccharomyces cerevisiae of 0.002 g/mL and a third combined diet at a ratio of 50%. Once the cultures were established, a greater population growth was evidenced in T3 (2417±15.28), followed by T1 (1790±36.06) and T2 (613±6.43), in the same way, the population growth rate and the doubling rate shown that T3 was the best, according to the analysis of variance there was a significant difference (p<0.05) between the treatments. Using the Lowry method, the protein percentage was extended, obtaining a higher protein percentage in the treatment (T3) with 68% protein, compared to (T1) 60% and (T2) 14%, subsequently , three different diets were tested in zebrafish (Danio rerio) in the juvenile stage, when supplying the combined diet (T3) the results were positive in the development of zebrafish, with an average length of (3.55cm ± 0.12) and weight ( 0.40g±0.03), according to the analysis of variance, there was no significant difference between T1 and T2, on the other hand, the maximum weight and length gain was dissolved in T3 at 45 days, in T1 and T2 the weight gain is the same at 30 days, in T1 at 30 and 45 days the length gain is greater than T2. It is concluded that the culture of Moina sp can be used as food for the nutrition of zebrafish, (Danio rerio), due to its nutritional contribution and the favorable response in the fish, in addition, using a commercial diet enriched with Moina sp improves the growth rates of the fish.