Evaluación de dos protocolos convencionales para la extracción de ADN humano a partir de hisopados bucales

Abstract

DNA extraction has been performed by different techniques ranging from conventional methods to commercial kits, but the latter represent high costs and it is essential to find an efficient method to obtain viable samples for molecular diagnosis. For this reason, two conventional protocols for extracting DNA from buccal swabs were evaluated. The extraction results were compared with the phenol/chloroform and salt precipitation methods, with protocol 1 extracting the greatest amount of DNA and protocol 2 extracting the highest quality DNA from the samples analysed by 0.7% agarose gel electrophoresis.