Evaluación de la actividad de enzimas lignolíticas producidas por el hongo (Pleurotus ostreatus) cultivado en residuos de caña de azúcar.

Abstract

In In this experimentation the enzymatic activity of Pleurotus ostreatus was evaluated, also known as white rot fungi, these are capable of acting in the degradation of lignin and other phenolic molecules that can be potential pollutants. Sugar cane waste (bagasse) was used as a substrate for the growth of P. ostreatus, necessary for lignocellulosic enzymatic development; laccases, lignin peroxidases and manganese peroxidases. Enzyme activity was compared between culture media enriched with calcium carbonate (CaCO3) and ammonium sulfate (NH4) 2SO4 with an unconditioned substrate used as a control. To establish the enzymatic activity, the ABTS method for laccase was used by spectrophotometry, the ABTS method modified by the author was used for lignin peroxidase, and for manganese peroxidase the oxidation of (MnSO4), modified by the author, was used. Enzyme production was analyzed for five days 7, 14, 21, 28 and 35. The results denote the highest enzymatic production of laccase on day 35; for manganese peroxidase the maximum activity was reached on day 7, while the production of lignin peroxidase was not evidenced, with the enriched treatment during the experimentation days.