Determinación de la actividad enzimática de manganeso peroxidasa en microorganismos presentes en el suelo del páramo del volcán Chiles, provincia del Carchi, para evaluar su potencial como biodegradadores de plástico polietileno (PE)

Abstract

This research aims to find an alternative to reduce waste plastic polyethylene using microorganism’s manganese peroxidase enzymatic activity to polyethylene biodegradation. 56 strains of microorganisms were isolated divided by different macroscopic characteristics from a total of 9 subsamples. These subsamples were taken at an altitude of 3 600, 3 800 and 4 000 meters above sea level (masl) of the Chiles volcano located in Tufiño parish, Carchi province. The planting of soil samples was done by sowing on the surface technique using Sabouraud Agar, Dextrose Potato Agar, and Trypticase Soy Agar, the isolated strains were developed at 25 °C for an incubation time of 5 days. The presence of manganese peroxidase enzymatic activity was determined by phenol red oxidation method, obtaining activity values ranging from 0.54 U/L to 7.84 U/L. Besides, the specific activity was determined. It allows knowing the specific amount of manganese peroxidase enzyme per milligram of protein establishing values ranging from 0.32 to 10.11 U/mg of protein. The highest manganese enzymatic activity peroxidase by microorganisms was five: SB-3800-S1-C22 with value of 3,05 U/L, PDA-3800-S3-C35 with value of 3,14 U/L, SB-3800-S3-C29 with 4,20 U/L, SB-3800-S1-C53 with 5,09 U/L and finally SB-3800-S2-C27 with activity value of 7,84 U/L. The strains that exhibited the highest enzymatic activity are potential plastic biodegrades.