Análisis químico mediante técnicas cromatográficas y espectroscópicas de los antibióticos producidos por las bacterias Micrococcus luteus Y Streptococcus sp., aisladas del suelo

Abstract

This research is based on the extraction, characterization and analysis of antibiotics produced by Micrococcus luteus and Streptococcus sp. encoded as PAP49G3A and PAP48 respectively, using techniques such as: Infrared Spectrophotometry (IR) where amino functional groups were visualized, which confer antimicrobial capacity, with thin layer chromatography (TLC), the antibiotic fraction was separated and subsequently analyzed by mass spectrophotometry technique, with the mass spectrophotometry the molecular weight of 92.31 mg/mol for PAP48 was known, and the molecular weight of 93.31 mg/mol for PAP49G3A, thus assembling the possible chemical structure of each antibiotic; With tests of antimicrobial activity such as: Minimum Inhibitory Concentration it was verified that PAP48 at a concentration of 300 mg / mL inhibits the growth in 88.70 % of Bacillus spizizenii and 86,60 % in Pseudomonas aeruginosa, while PAP49G3A at a concentration 300 mg / mL inhibits growth in 91,46% of Bacillus spizizenii and 89,88 % in Pseudomonas aeruginosa; using the Bioautography technique, the mode of action of antibiotic extracts against control pathogenic microorganisms was identified by inhibiting their growth.