Evaluación de la actividad antimicrobiana y antiinflamatoria de flavonoides presentes en las flores de Dalea coerulea (L.f.) Schinz & Thell.

Abstract

The purpose of this research was to verify the traditional uses of Dalea coerulea (L.f.) Schinz & Thell, which is used as a useful plant in diseases of the respiratory system. The flowers are used as a medicinal part, so their qualitative and quantitative chemical composition was determined, where the most representative molecules were the flavonoids. The highest composition of poliphenolic compounds and total flavonoids was found in the extract got with 50% ethanol, with the following values: total phenols 15,56 mg/g and total flavonoids 11,73 mg/g. The presence of malvidin was determined by mass spectrometry. To guarantee the ancestral use, the antimicrobial activity in the extracts was investigated in several microorganisms that cause respiratory system infections with the following results of Minimum Inhibitory Concentration: Haemophilus influenzae ATCC 10211TM MIC 328,947 μg/mL; Staphylococcus aureus subsp. aureus ATCC 29213TM MIC 367,647 μg/mL; Streptococcus pyogenes ATCC 19615TM MIC 367,647 μg/mL; Pseudomona aeruginosa ATCC 9027TM MIC 416,666 μg/mL y Klebsiella pneumoniae subsp. pneumoniae ATCC 10031TM MIC 1666,66 μg/mL. Additionally, the anti-inflammatory potential was determined, using the methodology of plantar edema induced by carrageenan, in laboratory animals, with doses of: 200, 100, 50 and 25 mg/kg of lyophilized extract D2 (got with 50% ethanol), The best result was at 300 minutes by applying a dose of 200 mg/kg, with a 46,49% of inflammation.