Identificación molecular de hongos endófitos y micorrízicos presentes en la relación simbiótica de la especie Caucaea pichinchae (orchidaceae) con su respectivo hospedero.

Abstract

In the present work the endophytic and mycorrhizal fungi of the Caucaea pichinchae species will be studied, with their respective host in the four collection points distributed in the Province of Pichincha, for the molecular study the leaves and the root of C. pichinchae were taken and Oreopanax ecuadoriensis, known as "Pumamaqui", for the isolation of the fungal species were established crops in nutritious medium PDA, this material was used to extract DNA, from which the ITS nuclear segment was amplified. For the identification of the host and guest, DNA was extracted from leaf tissue and a segment of the chloroplastic matK gene was amplified. The obtained sequences were sequenced and used for the identification of the species, in addition the familiarity of the species was analyzed by phylogenetic analysis; We identified 25 species of endophytes present in the stem of the host, of which one species is present in three of the four localities studied, in relation to the samples isolated from the guest root, 18 fungal species were identified, of which two species were repeat in two locations; the other species are specific to each collection site. In conclusion, the species Caucaea pichinchae establishes fungal populations mostly different in each sector, very rarely are repeated in locations.