Evaluación de la producción de ficocianina a partir de Arthrospira platensis en medios de cultivos zarrouk, jordan y medio artesanal.

Abstract

In this titration work, the effect of different compositions of Zarrouk, Jordan and Artesanal culture media on the growth and phycocyanin production of the cyanobacterium Arthrospira platensis was evaluated using seawater as a control. Growth curves were presented and elaborated with data from the Sedgwick-Rafter chamber for cell counting, which was determined during one month, evaluated three days a week. The percentage of phycocyanin of this cyanobacterium was measured by formula and cell rupture techniques, obtaining the sonicator technique for 20 min and 12 hours of slow freezing as the procedure to obtain the best result. Samples should always be mobilized covered with aluminum foil to avoid photo degradation. Two measurements were performed, one intermediate and one final, all in duplicate to avoid significant error in a cyanobacterial death stage. The assay was performed with a completely randomized DCA model. It is concluded that Arthrospira platensis, has a good growth and production of phycocyanin in all the proposed treatments, however, the artisanal treatment would be the best option since it could be noted by cell counting a greater number of filaments, as well as a percentage of pigment of 14.26%, thus concluding that the concentrations of phycocyanin were higher in the Artisanal and Zarrouk treatments and would be a viable option. The results obtained in the statistical program Infostat indicate that there were no significant differences (p>0.05) with respect to the percentage of phycocyanin between the treatments of the microalgae cultivated under the same laboratory conditions. While the result of the Excel growth factor analysis showed that there were differences between the treatments with respect to cell division, corroborating that the treatment with the highest cell division was the one with the highest number of cells. The results of the Excel growth factor analysis showed that there is a difference between the treatments with respect to cell division, corroborating that the treatment with the highest division and amount of pigment is the handmade medium, and also concluded the importance of evaluating phycocyanin 15 days after its development.