Tamizaje fitoquímico y actividad antimicrobiana de los extractos acuoso y alcohólico de las raíces de almacenamiento de Eriotheca Ruizii

Abstract

The characterization of plant species with little research on the phytochemical composition gives the opportunity to discover new and valuable compounds, allowing to know their properties in order to offer sustainable alternatives for the development of products with industrial applications of interest, scientifically validating at the same time the ancestral knowledge of communities. The purpose of this research work was to determine the qualitative content of the storage roots of Eriotheca ruizii through phytochemical screening of the aqueous, alcoholic and hydroalcoholic extracts. The most representative secondary metabolites were alkaloids, carbohydrates and flavonoids, in addition, Glyceryl oleate was determined as the compound with the greatest similarity following the technique of Fourier Transform Infrared Spectrometry (FT-IR) with a similarity percentage of 88.26%. The antibacterial activity was verified based on the micro dilution technique where a MIC of 1800,000 μg/mL was obtained against the S. aureus strain and 2076,923 μg/mL for E. coli, classifying it as "Moderate" antibacterial activity for both alcoholic and hydroalcoholic extracts at 20% concentration. For the evaluation of the antioxidant capacity of the extracts was used the DPPH method, whereas the best results were obtained for the hydroalcoholic extract with an IC50 of 150.8 ug/mL, considered as "Medium" antioxidant activity.