Evaluación de cuatro medios de cultivo para el aislamiento, purificación y crecimiento de haematococcus pluvialis

Abstract

Microalgae attract the interest of various industries due to the discovery of its various applications and benefits. Among them, Haematococcus pluvialis stands out, a source of the antioxidant known as “astaxanthin”, as well as other important bioactive molecules for human and animal health and nutrition. The purpose of this work was to evaluate the capacity of the modified Bristol, Rubic, BG11 media, and a proposed artisanal medium, for the optimal growth of H. pluvialis, in which the striated isolation technique was applied in solid media and as a purification technique, the same liquid consistency media were used, where growth kinetics and morphological changes for this microalga were monitored; in the experimental units in 10 mL tubes under conditions of 12 hours of light and 12 hours of darkness and a temperature of 20 °C, for a period of 21 days, versus the same liquid media in 100 mL Erlenmeyer flasks for a period of 12 days with the following conditions: stirring at 140 rpm, temperature of 20 °C and with the same photoperiod mentioned above. The results presented by means of the single factor ANOVA analysis of variance method (P < 0.05) indicate that there is a difference in the growth of H. pluvialis between the treatment of the four media in both flasks and tubes. The best culture medium turned out to be the artisanal medium, which obtained 1.38 x105 cells/mL in flasks and 7.4 x105 cells/mL in tubes, thus being the most suitable medium for the isolation, purification, and growth of the microalgae.