Desarrollo de un protocolo para el cultivo in vitro de la especie Caucaea Pichinchae Orchidaceae.

Abstract

This research aimed to conserve the Caucaea pichinchae species, through the development of in vitro culture techniques, in order to have the genetic material necessary for its study and commercialization. The plant material was collected in march on the slopes of the Corazón volcano in the Mejía canton in the Umbría protective forest, using mature capsules, employing the tetrazolium technique whose viability test was 96.5%, later prepared the three culture media: M1: Knudson plus medium; M2: Middle Vacin and Went; M3: Lindeman, maintaining a pH of 5,8 to favor the subsequent absorption of nutrients, in terms of the preparation of gibberellins, three concentrations of 0,05, 0,1 and 0,2 mg/L were used, from a 1 ppm stock solution. For the disinfection of the seeds, a 10 mL syringe was used with a piece of cotton that retained the seeds, with a 3% hypochlorite solution supplemented with two drops of tween, then they were rinsed with distilled water, after these two drops per test tube were inoculated on the culture media, dispersing uniformly so that they cover the surface well, the process was carried out inside the laminar flow chamber under aseptic conditions. The results showed that the M2 culture medium with the concentration of gibberellins (GA3) at 0,1 mg/L, presented a greater number of protocorms, for which it was possible to conclude that this phytohormone increases the amount of protocorms by inducing cell division at a higher rate. low concentration, and that the Vacin and Went medium can be used to obtain a higher percentage of protocorms of the species, together with a photoperiod of 16 hours of light and 8 hours of darkness.