Evaluación del proceso de biorremediación de suelos contaminados por hidrocarburos totales de petróleo utilizando Trichoderma sp. Y Bacillus pumilus, mediante el sistema de biopilas a escala de laboratorio

Abstract

This research addressed the biodegradation process of soils contaminated by TPH (total petroleum hydrocarbons), because of the serious impact that this causes in the environment, the difficult restoration, and the high costs of decontamination. The main objective of this research was to evaluate the TPH removal capacity using a biopile system with Trichoderma sp. and Bacillus pumilus. For the extraction and quantification of TPH, the methodologies EPA's 3540C SOXHLET EXTRACTION were used and 8015B NONHALOGENATED ORGANICS USING GC / FID respectively. By means of a statistical analysis a DCA in factorial arrangement 32 and a Tukey test at 5% were used to evaluate the best of the treatments. According to the analysis carried out, it is concluded that the remediation percentages of TPH, for a concentration of 10,000 ppm they were: with Trichoderma sp. a removal percentage of 65.15% and with Bacillus pumilus a percentage of 50.68%. On the other hand, for a concentration of 40000 ppm, they were: with Trichoderma sp. a removal percentage of 18.63% and with Bacillus pumilus a percentage of 18.67%, the best treatment with the highest percentage of removal is using Trichoderma sp. at a concentration of 10000 ppm.