Cultivo in vitro de Scoparia dulcis L. (Scrophulariaceae)

Abstract

In the framework of this investigation the techniques were designed for the propagation of meristematic tissue present in the bud of the nodal segments of the species, Scoparia dulcis L. (Scrophulariaceae), thereby obtaining plants genetically identical to the mother plant. They were identical to the plant mother.These techniques help to reduce the space and time necessary for the cultivation this species. The protocol thus developed will produce specimens of high performance in active principles and free of infectious agents, particularly for the production of material required for medicinal products. Scoparia dulcis L. has been described with anti-malarial proprieties. One of its chemical components is beta sitosterol, which is used to cure prostate inflammation and constitutes a base element for the treatment of baldness. The research was developed in several stages: first, the establishment of a protocol for bacterial and mushroom disinfection of explants. With this purpose the times of explants exposition to sodium hypochlorite 2,25% and 3% were tested. The second stage was to determine the ideal in vitro cultivation environment. The basic environment was Murashige and Skoog, to which several growth hormones (IBA, ANA y 2,4-D) were added. The last stage was to test the adaptation of the species to a rustic low-level control environment. Explant disinfection was conducted in sodium hypochlorite 3% for 23 minutes. in vitro cultivation required a basic environment of Murashige and Skoog without growth hormones.